Contralateral flank experiments
After restimulation, TRM cells may facilitate enhanced recruitment of memory T cells from the circulation12. We examined whether … See more To understand how TRM cells directly control viral infection at the point of pathogen entry, we activated congenically marked CD45.1+CD8+ gBT-I transgenic mouse T cells specific for the immunodominant … See more We next asked whether epidermal TRM cells can migrate toward foci of infected keratinocytes as well as into the underlying dermis. To distinguish between gBT-I TRM cells and gBT-I T cells recruited from the … See more To examine how skin TRM cells mediate protection after infection, we transferred in vitro–activated EGFP+ gBT-I T cells into mice and then applied DNFB to induce skin lodgment. More than 30 d after EGFP+ gBT-I T cell … See more Phenotypic flow cytometry analysis of gBT-I TRM cells isolated from previously challenged skin >30 d after HSV infection or DNFB treatment … See more WebApr 12, 2024 · ( A) Frequency of epidermal LCs (of total single epidermal cells) from skin surrounding tumors or unrelated contralateral skin at indicated time points after injection of YUMM1.7 cells. ( B) Frequency of LCs (of total single LN cells) in tumor-draining LNs or uninvolved LNs at different time points after tumor injection.
Contralateral flank experiments
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WebFlank tumor rechallenge experiments were performed with tumor-free mice approximately 40 days after treatment start unless otherwise indicated. Rechallenge consisted of intradermal injection of 2×10 6 tumor cells on the contralateral flank from the original tumor cell injection. Tumor volume was assessed twice weekly and mice were euthanized ... WebAug 12, 2009 · Representative of two independent experiments. B, GFP + IKDCs were injected into the contralateral flank of mice bearing 10-d established tumors. Tumors and DLNs were harvested 24, 48, or 72 h …
WebIn double-flank transplantation experiments, CT26WT tumors grew despite successful eradication of CT26LacZ tumors from the contralateral flank. Tumor growth progressed … WebOct 11, 2024 · In one model, two contralateral flank tumors were established subcutaneously in mice. In subsequent experiments, tumor cells were injected into the …
WebJun 3, 2024 · In the distant contralateral TNDLN, the percentage of DC subsets was increased in both cryoablation groups as compared to controls. These results suggest … WebApr 19, 2024 · (A, C, and F) Data pooled from two independent experiments, n = 5 to 10 mice/strain ± SEM, two-tailed unpaired Student’s t test, except D, linear regression with 95% CI (dotted lines). ... Fig. S4A), then CCL19 and CCL21 were measured in grafts and contralateral-flank host skin following a 6 wk recovery period to permit lymphatic repair .
WebNov 23, 2015 · As a surgical sham control, mice underwent a 1 cm skin incision and closure in the contralateral flank without manipulation of the tumor site. In selected experiments, partial resection was performed, classified by detectable macroscopic and microscopic residual disease in which ~50% of the tumor mass was excised.
WebJul 19, 2024 · Flank tumor growth analysis demonstrated an average mouse receiving ISV had an additional 20% decrease (95% CI 18% to 21% reduction, p<0.001) in geometric … tarek and christina el moussa sell homeWebJul 23, 2013 · In a typical experiment, synthesis of hollow gold nanoshells were prepared by first aging an aqueous solution of 0.2 mM silver nitrate ... After 6 days, mice were injected with the same tumor on the contralateral flank. When the primary tumors reached 5–8 mm (day 12–14), they were treated with PTT and the subsequent growth of both treated ... tarek and christina new showWebApr 1, 2024 · The left flank is more difficult for a right-handed person to inoculate, because most researchers are trained to scruff the mouse with the left hand and inoculate the right flank. tarek and christina get divorcedWebSep 23, 2024 · When tumor size reached 3–5 mm in diameter, tumor-bearing mice were injected intratumorally (intratumoral N. caninum injection group) or subcutaneously in the contralateral flank (distal subcutaneous N. caninum injection group) with 2 × 10 6 N. caninum tachyzoites suspended in PBS or PBS (tumor control group), respectively, as … tarek and christina newsWebMay 4, 2024 · Make sure that the skin in the flank is not pulled tight. 9. Person 2: Lift the skin in the flank to create a subcutaneous space for injection. Insert the needle, inject … tarek and christina home listingsWebApr 12, 2024 · In the experiment with flat prosody, a significant cluster emerged only in the LH, we therefore took the corresponding cluster and time window in the RH as the contralateral ROI. We used normalized concentration changes in the ANOVA, as is appropriate for between-subject comparison with NIRS data ( 17 , 40 , 41 ). tarek and christina houseWebTumor formation in ipsilateral and contralateral flanks. Genetically modified B4B8 cells (5106) were injected subcutaneously into the left flank (ipsilateral) and naïve B4B8 cells … tarek and christina realty