Ctll-2 ed50

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August undefined, 2024

WebED50 is < 10 ng/mL as determined by the dose-dependant stimulation of the proliferation of CTLL-2 cell, corresponding to a specific activity of > 1 x 10 5 IU/mg. Recommend Usage: Biological Activity ... Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. ... WebInterleukin-2 (IL-2), originally called T cell growth factor (TCGF), is a multifunctional cytokine which can stimulate the differentiation and proliferation of T lymphocytes and other cell types including B lymphocytes, NK cells, LAK cells, and monocytes/macrophages. ... ED50 = 10 - 150 pg/mL, has previously been reported using CTLL-2 as ...

Rat IL-2, Animal-Free Recombinant Protein (AF-400-02-1MG)

WebThe ED50 as determined by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells is ≤ 0.4 ng/ml, corresponding to a specific activity of ≥ 2.5 x 10^6 units/mg. ... natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also ... WebMeasured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. ... The ED50 for this effect is 0.05-0.25 ng/mL. The specific activity of Recombinant Human IL-2 is approximately 9.1 x 106 IU/mg, which is calibrated against recombinant human ... disability and the elderly

CTLL-2 - TIB-214 ATCC

WebAliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. Activity ED 50 = 0.3 - 1.8 ng/ml, corresponding to a specific activity of 0.55 - 3.3 x 10 6 units/mg, as determined by CTLL-2 cell proliferation induced by mouse IL-4 in a dose dependent manner. WebBioactivity: The ED50 of this protein, as determined by increased proliferation of mouse CTLL-2 cells stimulated with 1 ng/mL IL-2, is 0.5-1.5 µg/mL. Endotoxin: Less than 0.1 … WebCTLL-2 cell proliferation: ED50 ≤ 5 ng/mL: Preparation and Storage Country of Origin: USA Shipping: Room temperature Storage Prior to Reconstitution: -20°C Product Reconstitution: Sterile 10 mM sodium bicarbonate, pH 8.5 at 0.1 mg/mL Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended ... fotobearbeitungs app windows 10

Mouse IL-2 Recombinant Protein (RP-8605) - Thermo Fisher Scientific

ctll-2 Cells Thermo Fisher Scientific - US

WebMeasured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.0300-0.250 ng/mL. Source. E. coli-derived human IL-2 protein Ala21-Thr153 (Cys145Ser), with and ... WebRecombinant Human Interleukin-2(IL2),Partial (Active) Supplier Page. Supplier Page from Biorbyt for Human IL2 protein. Product Specs; ... The ED50 as determined in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells is less than 0.5 ng/ml. disability and transportationWebData Figures. (A) The biological activity of Human Recombinant IL-2, ACF was tested by its ability to promote the proliferation of CTLL-2 cells. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 1.99 ng/mL. (B) 1 μg of Human Recombinant IL-2 ... foto bearbeitungs app kostenlos windows 10

"WebED50 ≤ 5 ng/mL; determined by the dose-dependent proliferation of CTLL-2 cells. Conjugate: Unconjugated : Form: Lyophilized: Concentration: 0.1 mg/mL: Purification: purified: ... Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells ... " - Ctll-2 ed50

Ctll-2 ed50

Rat IL-2, Animal-Free Recombinant Protein (AF-400-02-1MG)

WebJun 2, 2012 · FIGURE 61.1. A bioassay for IL-2 using CTLL-2 cells. Supernatants of tumor cells (PCl-13) transduced with the gene for human IL-2 were collected and tested for the … WebBioactivity: The ED50 of this protein, as measured by CTLL-2 cell proliferation assay, is less than or equal to 4 ng/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 10e5 Units/mg. Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: 29.4 kDa.

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WebCTLL-2 cells were seeded in a 96-well plate and treated with varying concentrations of Human Recombinant IL-2. Cell proliferation was measured using a colorimetric assay. In the graph below, the specific … WebED50 : 0.5 ng/mL; determined by the dose-dependen proliferation of mouse CTLL-2 cells. Conjugate: Unconjugated : Form: Lyophilized: Purification: Size-exclusion - Gel filtration: Contains: ... Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural ...

Web>2 x 106 units/mg Example calculation with Gibco™ Human IL-2 Recombinant Protein (Cat. No. PHC0021) Note: If the ED 50 is expressed as a range, use the midpoint of the range. … Webctll-2 Cells. Organism Type. Mouse. Cytotoxic T cell line (CD8+) Subculturing: Subculture actively growing suspension cultures before they the reached 1 X 10 exp5 cell/ml. Use …

WebED50 : 0.0645 ng/mL; determined by dose-dependent stimulation of murine CTLL-2 cells. This corresponds to a specific activity of 1.55x10e7 IU/mg. Conjugate: Unconjugated : ... The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is 0.0645 ng/mL, corresponding to a specific activity of 1.55x107 IU/mg. Purity: >99%. WebBiological Activity: The ED50 as determined by its ability to block human IL-15-induced proliferation of CTLL‑2 mouse cytotoxic T cells is less than 10 ng/ml. Sequence ITCPPPMSVE HADIWVKSYS LYSRERYICN SGFKRKAGTS SLTECVLNKA TNVAHWTTPS LKCIRDPALV HQRPAPPSTV TTAGVTPQPE SLSPSGKEPA …

WebED 50 =2.5 - 15 ng/ml, corresponding to a specific activity of 0.6 - 4 x 10 5 units/mg, as determined by the dose dependent stimulation of CTLL-2 cell proliferation. Application …

WebIL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. ... Determined its its ability to … disability and the mediaWebMar 25, 2024 · Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. ... The ED50 for this effect is 0.05-0.25 ng/mL. The specific activity of recombinant human IL-2 is 5.0 x 106 IU/mg, which is calibrated against the human IL-2 WHO International ... disability and wellbeing qubWebThe newly developed cell lines (termed CTLL-2_NF-kB and A20_NF-kB) were used to implement a general bioassay, which was compared to established procedures for the characterization of various types of engineered cytokine products. Moreover, the new methodologies allowed the discovery of a novel strategy for cytokine potentiation, based … disability and votingWebBioactivity: ED50 is determined by the dose-dependent Proliferation of CTLL-2 cells. The ED50 is typically less than 1 ng/mL. The international units of CTGrade rh IL-15 is approximately 2.7 x 10. 5; IU/µg, which is calibrated against recombinant Human Interleukin 15 … fotobearbeitungsprogramm gratis windows 10Web一种重组人il2包涵体纯化复性方法技术领域 1.本技术涉及一种重组人il2包涵体纯化复性方法，属于蛋白表达纯化技术领域。背景技术： 2.白细胞介素2(il2)又称t细胞生长因子，是由t细胞(cd4+和cd8+)和nk细胞产生，主要受抗原和t细胞受体结合刺激分泌。 il2基因位于染色体4q26-28,长度3662bp,含4个外显子和3 ... fotobearbeitungs app windowsWebEach lot has its own ED50 determined using an activity assay based on a specific function of the growth factor. The activity of our IL2 CTS™ Recombinant Human growth factor is … fotobearbeitungsprogramm freeware windows 10WebIL2, IL-2, TCGF, lymphokine. Bioactivity. The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. Purification Method. SDS-PAGE & … foto bearbeitungs programm von windows