WebKonjac (Amorphophallus konjac K. Koch) is a well-known tuberous vegetable belonging to the important medicinal family Araceae, and the plant grows from an underground tuber. Here, we used a “one-step seedling regeneration” tissue culture system to improve the plantlet regeneration efficiency of konjac using young leaves as an explant source. In … Web• Inoculation into the deeper layers of solid media, or under a layer of oil in liquid media or • A combination of the above methods. Moisture Proper moisture conditions must prevail in the culture media for the growth of microorganisms. A moist medium and atmosphere are necessary for the continued luxuriant growth of cells.
PREPARATION OF MEDIA I. OBJECTIVES II. INTRODUCTION
WebJun 13, 2024 · An example method of the present disclosure comprises imbibing a Cannabaceae seed in a hydration solution, excising a subset of embryonic tissue from the imbibed Cannabaceae seed to extract a Cannabaceae meristematic region or embryonic axis (EA), and exposing the Cannabaceae meristematic region or EA to a heterologous … Webcontext, use of healthy seed of recommended varieties through hot air treatment / and shoot tip culture technique becomes exceedingly important. The conventional mode of seed multiplication has a multiplication rate of 1: 8-10. As a result, a new variety takes 7-8 years to saturate the command area. A much lewis cll written works
Laboratory Manual Principles of Plant Biotechnology
WebThe term explant can be applied to samples obtained from any part of the organism. The extraction process is extensively sterilized, and the culture can be typically used for two … WebThis improved method resorts to Agrobacterium-mediated transformation of the split-seed explant with an attached partial embryonic axis obtained from an imbibed seed. In addition to the split-seed explant, Agrobacterium strain and preparation were shown to be important for improved transformation. WebWhat is inoculation and its purpose? Inoculation may be defined as the process of adding effective bacteria to the host plant seed before planting. The purpose of inoculation is to make sure that there is enough of the correct type of bacteria present in the soil so that a successful legume-bacterial symbiosis is established. mcc neet