Tīmeklis2024. gada 9. marts · EZH2 (Roche-SP219), PTEN (CST-138G6). Purification of epithelial cells from breast tissue The protocol followed for purification of organoids … Tīmeklis2024. gada 3. janv. · As described in the guide, cell lysate was incubated with beads coated with antibodies against EZH2 (CST, #5246S) and H3K27me3 (Abclonal, A2363). The bound DNA fragments were eluted and measured by RT-qPCR to determine the enrichment of EZH2 or H3K27me3 in the promoter region of the target genes.
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Tīmeklis2024. gada 15. jūn. · Primary antibodies (YAP = CST-12395, YY1 = CST-63227, and EZH2 = CST-5246) were used at 1:600. Images were taken on an Olympus FV3000RS confocal scanning microscope. DAPI was visualized at 405 nm, and Duolink red visualized at 594 nm. ChIP-seq data analysis. TīmeklisFigure 3 . Knockdown of EZH2 reduces apoptosis and inflammatory responses in vitro. (A) Expression of EZH2, cleaved-caspase3, Bax, and Bcl2 in HK-2 cells with or without shEZH2 was detected by western blotting and quantified by densitometry.(B)The levels of TNF-α, IL-6 and IL-1β were determined by qPCR. (C) Cell activity was determined … hubert harned towboat
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Tīmeklis2024. gada 27. nov. · Hsu et al. describe the discovery of EED-targeted PROTACs that lead to the degradation of multiple components of the PRC2 complex. Furthermore, they demonstrate that these PROTACs inhibit the proliferation of PRC2-dependent cancer cells and may be considered an attractive alternative to EZH2 catalytic inhibitors … TīmeklisPolyclonal Antibody for studying EZH2. Cited in 85 publications. Validated for WB, IP, ChIP. Highly specific and rigorously validated in-house, Ezh2 Antibody (CST #4905) … TīmeklisPolyclonal Antibody for studying EZH2. Cited in 85 publications. Validated for WB, IP, ChIP. Highly specific and rigorously validated in-house, Ezh2 Antibody (CST #4905) … hogwarts legacy pre order release time est