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Lamp2b-ha

Tīmeklis2024. gada 4. jūn. · In this approach, exosome-producing cells were transiently transfected with a pcDNA GNSTM-3-RVG-10-Lamp2b-HA vector to express Lamp2b fusion protein fused in frame with the neuron-specific rabies ... TīmeklisSequences pcDNA GNSTM-3-RVG-10-Lamp2b-HA Sequences (3) Addgene Sequences: Partial (2) Depositing Scientist Sequences: Full (1) Full Sequences from …

Mouse brain-wide transgene expression by systemic injection of ...

Tīmeklis2024. gada 24. dec. · Mutations in lysosomal-associated membrane protein 2 ( LAMP-2) gene are associated with Danon disease, which often leads to cardiomyopathy/heart failure through poorly defined mechanisms. Here, we identify the LAMP-2 isoform B (LAMP-2B) as required for autophagosome–lysosome fusion in human … Tīmeklis2024. gada 10. nov. · To produce sEVs RVG -siRNA, a recombinant vector, pcDNA-RVG10-Lamp2b-HA, was constructed and transfected into human embryonic kidney (HEK) 293T cells. The secreted sEVs were isolated from the culture supernatants by differential ultracentrifugation, as previously described. 16 handheld shower head grohe https://phillybassdent.com

pcDNA GNSTM-3-RVG-10-Lamp2b-HA Addgene 71294 product …

TīmeklispcDNA Flag Lamp2b-HA Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b. Loading... TīmeklispcDNA GNSTM-3-Flag-10-Lamp2b-HA (Plasmid #71293) Print Enlarge View all sequences Purpose Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag. Depositing Lab Joshua Leonard Publication TīmeklispMEH60 Lamp2b MS2 HA (Plasmid #168209) Print Enlarge View all sequences Purpose Mammalian expression of lysosome-associated membrane protein 2b fused … bush forest dora\u0027s house

Stabilization of Exosome-targeting Peptides via Engineered ...

Category:pcDNA GNSTM-3-Flag-10-Lamp2b-HA - biofeng.com

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Lamp2b-ha

Addgene: pMEH31 Lamp2b HA

Tīmeklis2016. gada 13. maijs · Human Lamp2b cDNA clone 3543019 was purchased from Open Biosystems (now sold by Dharmacon) and inserted into pcDNA3.1+ Hygro (Clontech). A C-terminal GSG spacer and an HA peptide tag (YPYDVPDYA) were added by PCR to create plasmid pMEH31. http://www.biofeng.com/adjy/zhili/pcDNA%20Flag%20Lamp2b-HA.html

Lamp2b-ha

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TīmeklispcDNA GNSTM-3-RVG-10-Lamp2b-HA (Plasmid #71294) Print Enlarge View all sequences Purpose Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, … TīmeklispcDNA GNSTM-3-RVG-10-Lamp2b-HA : 出品公司: 爱迪基因: 目录编号: 71294: 存储实验室: Joshua Leonard: 载体骨架基本信息: 载体名称: pcDNA 3.1+ Hygro: 载体出品 …

TīmeklispcDNA Flag Lamp2b-HA质粒价格1200元，pcDNA Flag Lamp2b-HA(爱迪基因#71292 )质粒图谱（map），质粒序列(Sequence)见下文，质粒抗性为氨苄青霉素，质粒大 … TīmeklisTo test this hypothesis, HEK293FT cells were transfected with Lamp2b containing a C-terminal HA tag (Lamp2b-HA), two C-terminal tags (Lamp2b-HA-FLAG), or an N …

Tīmeklis2015. gada 5. febr. · The Lamp2b fusion proteins were expressed at similar levels in cells, as indicated by the HA Western blot. However, the FLAG peptide on FLAG-Lamp2b-HA could not be detected, although the C-terminal FLAG peptide of Lamp2b-HA-FLAG was detected ( Fig. 1E ). The same pattern was observed in exosomes ( … TīmeklisWestern blot analysis shows expression of fusion protein CAP-Lamp2b-HA in total protein isolated 36 hours after electroporation of CAP-Lamp2b with BM-MSCs as both the Hemagglutinin HA-tag antibody specific and Lamp2b-specific positive signal shows in the 1 st western blot run ( .

TīmeklisLamp2b ( LAMP2 Human) Use Tags HA Expression Mammalian Mutation Promoter CMV Availability Academic Institutions and Nonprofits only Enlarge pCAG-G-Flamp2 Plasmid #192782 Purpose green biosensor for cAMP in mammalian cells Depositor Jun Chu Article Liu et al Front Pharmacol. 2024 May 12;13:902290. Insert G-Flamp2 Use … handheld shower head holdersTīmeklis2024. gada 9. apr. · GNSTM-3-FLAG10-CAP-Lamp2b-HA plasmid, the expression of CAP-Lamp2b-HA fusion protein, we assessed the protein level of Lamp2b and CAP-Lamp2b-HA fusion protein in BM-MSCs 48 hours after electroporation. Compared with BM-MSCs electroporated by pcDNA-Hygro-Lamp2bplasmid, the western blotting … bush forestTīmeklispMEH31 Lamp2b HA: Lamp2b (Homo sapiens) Leonard Add to Cart 168209: pMEH60 Lamp2b MS2 HA: Lamp2b (Homo sapiens) Leonard Add to Cart 183645: pBoBi … handheld shower head in brushed nickelTīmeklisHA (C terminal on insert) Cloning Information Cloning method Restriction Enzyme 5′ cloning site NheI (destroyed during cloning) 3′ cloning site BamHI (destroyed during … bush for end of drivewayTīmeklisPlasmid pMEH31 Lamp2b HA from Dr. Joshua Leonard's lab contains the insert Lamp2b and is published in J Extracell Vesicles. 2016 May 13;5:31027. doi: 10.3402/jev.v5.31027. eCollection 2016. This plasmid is available through Addgene. handheld shower head leakingTīmeklis2024. gada 24. nov. · Lamp2b-HuR融合蛋白功能化的外泌体能够将被RNA结合基序识别的RNA靶标驱动至溶酶体以在受体细胞中降解。 该系统在富含溶酶体的巨噬细胞中 … bush forestry equipmentTīmeklis2024. gada 20. jūl. · The construction of pRBP-Lamp2b-HA has been previously reported. 36 HA is a commonly used epitope tag, which does not interfere with the … bush forestry