Lamp2b-rvg
TīmeklisLamp2 signal peptide (N terminal on insert) GNSTM glycosylation motif (N terminal on insert) Rabies Viral Glycoprotein (RVG) peptide targeting acetycholine receptor (N … Image: Illustrated plasmid map in PNG format GenBank File: Plasmid sequence … Plasmid Luciferase-pcDNA3 from Dr. William Kaelin's lab contains the insert … Tīmeklis2024. gada 4. aug. · A Lamp2b-based fusion protein can be used to display rabies viral glycoprotein (RVG), a neuron-specific targeting peptide, on the surface. Expression of Lamp2b-RVG fusion proteins in dendritic cells (DCs) displays RVG on the surface of DC-derived exosomes (Fig. 2 a).
Lamp2b-rvg
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Tīmeklis一项突破性的研究表明，狂犬病毒糖蛋白 (RVG)可以通过融合蛋白溶酶体相关膜糖蛋白2b (Lamp2b)-RVG，定位于EV的表面，实现脑特异性靶向的潜力。 作者利用同样的设计，制备靶向大脑的RVG-circSCMH1-EV，探讨circSCMH1在中风病理过程中的作用。 用编码GNSTM-RVG-Lamp2b-HA和circSCMH1的质粒共转染HEK293T细胞，然后进 … Tīmeklis2024. gada 24. aug. · 他们首先转导 DC 以表达外泌体膜蛋白 LAMP2B，该蛋白能与神经元特异性 RVG 肽融合。纯化的 DC 外泌体中装有靶向 BACE1（阿尔茨海默病的发病机理中的重要调控基因）的外源 siRNA，然后将这些 iExosome 静脉注射到小鼠体内。
Tīmeklis2015. gada 3. marts · This strategy has been applied to target exosome uptake by neurons by fusing a rabies viral glycoprotein (RVG) 3 peptide to the N terminus of lysosomal associated membrane protein 2b (Lamp2b) . Such a fusion resulted in RVG peptide being displayed on the surface of exosomes, leading to exosome uptake via … Tīmeklis2024. gada 1. dec. · A fusion protein of RVG and Lamp2b was expressed in 293T cells. Since Lamp2b is an exosome membrane-integral protein, RVG-Lamp2b is …
TīmeklisLAMP2. Lysosome-associated membrane protein 2 ( LAMP2 ), also known as CD107b ( C luster of D ifferentiation 107b) and Mac-3, is a human gene. Its protein, LAMP2, is … Tīmeklis2024. gada 10. janv. · To generate RVG-exo and T7-exo, stable cell lines were prepared by transfection of plasmids encoding RVG-Lamp2b and T7-Lamp2b into 293 T cells (Fig. 1 A).Then, isolation of ligand-decorated exosomes was confirmed by western blotting with an anti-HA-tag antibody because HA-tags were expressed as a fusion …
Tīmeklis2024. gada 24. nov. · Lamp2b-HuR融合蛋白功能化的外泌体能够将被RNA结合基序识别的RNA靶标驱动至溶酶体以在受体细胞中降解。 该系统在富含溶酶体的巨噬细胞中 …
Tīmeklis2024. gada 16. dec. · Abstract. Huntington's disease is an autosomal-dominant neurodegenerative disease caused by CAG expansion in exon 1 of the huntingtin (HTT) gene. Since mutant huntingtin (mHTT) protein is the root cause of Huntington's disease, oligonucleotide-based therapeutic approaches using small interfering RNAs (siRNAs) … settlor meaning in tamilTīmeklis2024. gada 15. dec. · The CMV-RVG-siR mHTT circuit was generated by fusing the rabies virus glycoprotein (RVG) tag into the extra-exosomal N-terminus of lysosome … settlor interested trust hmrcTīmeklis2024. gada 29. marts · Likewise, when C57BL/6J mice were injected with a circuit expressing an RVG-Lamp2b-eGFP triple fusion protein (RVG and eGFP were fused to the N- and C-termini of Lamp2b, respectively) to generate ... thetoasteranimatedkingstonTīmeklis2024. gada 21. okt. · The purified neuronal-targeted Lamp2b-RVG sEVs were subsequently loaded with SF1-AMPKα1-DN. The loading did not modify either the size or the morphological properties of the sEVs as measured by... the toasted yolk germantownTīmeklisRabies Virus Glycoprotein 是由 29 个氨基酸残基构成的细胞穿透肽，来源于狂犬病病毒糖蛋白，它能够穿过血脑屏障，进入脑细胞。 Rabies Virus Glycoprotein is a 29-amino-acid cell penetrating peptide derived from a rabies virus glycoprotein that can cross the blood-brain barrier (BBB) and enter brain cells. 细胞穿膜肽 穿透细胞膜进入细胞内是 … the toasted yolk cafe sarasotaTīmeklispcDNA GNSTM-3-RVG-10-Lamp2b-HA载体图谱质粒图谱、序列、价格、抗性、测序引物、大小等基本信息_生物风载体 pcDNA GNSTM-3-RVG-10-Lamp2b-HA 价格： … settlor interested trust minor childrenTīmeklis2015. gada 27. marts · Indeed, peptides fused to the N terminus of exosome-associated transmembrane protein Lamp2b were cleaved in samples derived from both cells … settlor interested trust tax