Phospho flow antibodies

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August undefined, 2024

WebPolyclonal Antibody for studying PLCg1 (Tyr783) phosphate. Cited in 265 publications. Validated for Western Blotting. Available in 3 sizes. Highly specific and rigorously … WebPhosphospecific antibodies are affinity-purified rabbit polyclonal or monoclonal antibodies that are monospecific for a target protein that is phosphorylated. These antibodies …

Recombinant APC Anti-p38 (phospho T180 + Y182) antibody …

WebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse … WebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse Monoclonal Antibody (CST #5282) is ready to ship. cynthia guerrini

Phospho-FLT3 (Tyr591) Antibody Cell Signaling Technology

WebPrimary Anti-ULK1 (phospho S556) antibody (ab203207) Primary Anti-AMPK gamma 1 antibody [Y308] (ab32508) Primary Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) View more … WebPhospho-specific antibodies allow for the phosphorylation state of individual protein residues to be determined by standard, immunoblotting techniques.26 Several phospho-specific antibodies have been developed for Raf-1, including antibodies that recognize phospho-Ser-338 and phospho-Tyr-340/341. WebPhospho-SMC1A-S957 Polyclonal Antibody cynthia grover jaws 2 movie images

Recombinant PE Anti-p38 (phospho T180 + Y182) antibody …

Anti-Phosphoserine antibody (ab9332) Abcam

WebThe antibody pair provided in this kit recognizes human Phospho-EGFR (pTyr1045). Storage Stability. Rapidly measure phosphorylated protein in lysates. Screen numerous different cell lysates without performing a Western Blot analysis. Minimal hands-on time, convenient, and non-radioactive material. WebKnockout Tested Rabbit recombinant monoclonal ZFP36L1 (phospho S92) antibody [EPR19926] - BSA and Azide free. Validated in WB, Dot and tested in Mouse, Human. cynthia guerardWebFlow Cytometry - APC Anti-p38 (phospho T180 + Y182) antibody [P38T180Y182-E3] (ab278728) Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using ab278728. billy\\u0026lilly

"WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. " - Phospho flow antibodies

Phospho flow antibodies

Protocol Compatibility for Immune Signaling and Phenotyping by Flow …

WebTermed "phospho-flow," these methods employ fluorescence-conjugated monoclonal antibodies that recognize phosphorylated epitopes of intracellular kinases, and may be combined with surface phenotypic markers to observe changes in … WebPhosphoserine Antibodies Antibodies that detect Phosphoserine can be used in several scientific applications, including Western Blot, Immunohistochemistry, ELISA, Immunoprecipitation and Dot blot. Our Phosphoserine monoclonal and polyclonal antibodies are developed in Mouse and Rabbit. Find the Phosphoserine antibody that fits …

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WebFor example, staining intracellular targets for phospho-flow cytometry experiments requires that you fix and permeabilize your cells before staining. However, fixation (fix) and permeabilization (perm) protocols can alter the interaction between epitope and antibody. WebApr 9, 2024 · “A Pan-specific Antibody for Direct Detection of Protein Histidine Phosphorylation” The idea is that the antibody is: ‘pan’ in that it will react with all phospho-histidine residues on proteins, but ‘specific’ in that it will not react with unphosphorylated histidine residues or other phospho-aminoacids e.g. phospho-serine. Share

WebAntibody Details Format Details Citations & References Brand: BD Phosflow™ Alternative Name: SMAD2, SMAD3; MADH2, MADH3; MAD homolog 2, MAD homolog 3 Reactivity: Human (QC Testing), Mouse (Tested in Development), Rat (Predicted) Isotype: Mouse IgG1, κ Immunogen: Phosphorylated Human Smad2 Peptide WebPhosphoflow is a specialized application of flow cytometry that enables researchers to examine the phosphorylation status of specific proteins that govern cellular responses …

WebJan 1, 2012 · Antibodies: 5. Anti-human and mouse phospho-STAT3 (pY705) mouse antibody conjugated to phycoerythrin (PE) (BD/Phosflow, catalogue number: 612569). 6. Anti-human and mouse phospho-STAT5a,b (pY694) mouse antibody conjugated to Alexa Fluor ® 488 (BD/Phosflow, catalogue number: 612598). 7. WebAll lanes : Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225) at 1/1000 dilution Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate Lane 2 : Jurkat treated with 50mM pervanadate for 5minutes whole cell lysate Lane 3 : Jurkat treated with 50mM pervanadate for 5min whole cell lysate 10 µg. Then the membrane was incubated with …

WebThis Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033. Product Usage Information Storage

WebThis antibody conjugate is suitable for intracellular staining of human cell lines and peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD Phosflow™ permeabilization buffers may be used. Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. cynthia guerrero ed.dWebAnti-NFAT1 (phospho S54) antibody See all NFAT1 primary antibodies Description Rabbit polyclonal to NFAT1 (phospho S54) Host species Rabbit Tested applications Suitable for: WB more details Species reactivity Reacts with: Mouse Immunogen Synthetic peptide corresponding to Mouse NFAT1 (phospho S54). Database link: Q60591 Positive control cynthia guerry toursWebAnti-Phosphoserine antibody See all Phosphoserine primary antibodies Description Rabbit polyclonal to Phosphoserine Host species Rabbit Specificity Recognize proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA. billy \u0026 emily england broWebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR1899 (2)] to JAK1 … cynthia guessWebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR1899 (2)] to JAK1 (phospho Y1034 + Y1035) - BSA and Azide free. Suitable for: ELISA, Dot blot, WB, IHC-P. Reacts with: Human. cynthia guerreroWebThis Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is … billy \u0026 mandy wcofunWebRabbit recombinant monoclonal p38 (phospho T180 + Y182) antibody [P38T180Y182-E3] conjugated to PE. Validated in Flow Cyt and tested in Rat. Immunogen corresponding to synthetic peptide. billy \u0026 the bruisers