WebMay 19, 2016 · Rbfox proteins regulate tissue-specific splicing by targeting a conserved GCAUG sequence within pre-mRNAs. We report here that sequence-specific binding of the … WebNotably, Rbfox gene structure in Drosophila already possessed two important features of the mammalian genes: an RRM coding domain, the core of which is contained within two …
The kinetic landscape of an RNA-binding protein in cells
WebFeb 9, 2024 · To examine how RbFox achieves high specificity, we first measured the affinity of the RNA-binding domain (RRM) of RbFox for two RNAs that differ by a single nucleotide … WebEach RBD/RRM has its own sequence preference and typically binds to a region of 2–6 nt in single-stranded RNA [15,18]. In this study, we found that the RBP Rbm38 suppresses the transcription elongation defect caused by splicing deficiency, and that the N- and C-terminal regions and the RBD/RRM domain of Rbm38 are required for this suppression. how to sell your property tips in austin
Splicing Activation by Rbfox Requires Self-Aggregation through Its ...
WebOct 23, 2024 · PDB Entry - 7VRL (Status - Released) Summary information: Title: Solution structure of Rbfox RRM bound to a non-cognate RNA DOI: 10.2210/pdb7vrl/pdb Entry authors: Yang, F., Varani, G. Initial deposition on: 23 October 2024 Initial release on: 26 October 2024 Latest revision on: 26 October 2024 Downloads: WebMay 5, 2024 · RBFOX1 is a member of the RBFOX family of RNA binding proteins that also includes RBFOX2 and RBFOX3. Rbfox1 expression is restricted to neurons, heart, ... At the … WebSep 11, 2024 · Rbfox family proteins contain two critical phenylalanine (F) residues within the RRM domains (amino-acids 108 and 142 in Rbfox3) that are required for RNA-binding activities. The F142 mutation to alanine (F142A) in Rbfox3 abrogates its binding activity to both the canonical binding motif UGCAUG and noncanonical target RNAs. how to sell your sister on ebay